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951.
Addition of tumor promoting phorbol esters, such as phorbol 12-myristate 13-acetate (PMA), to many cell lines results in a decrease of 125I-epidermal growth factor (EGF) binding and increased serine/threonine phosphorylation of the EGF receptor in a process termed transmodulation. It is, however, unclear whether or not receptor phosphorylation is causally related to the inhibition of high affinity EGF binding. We have investigated the significance of phosphorylation/dephosphorylation events in the mechanism of PMA-induced transmodulation using the adenylate cyclase activator cholera toxin and the serine/threonine protein phosphatase inhibitor okadaic acid. In Rat-1 fibroblasts treated at 37 degrees C, PMA induced a rapid decrease in EGF binding which persisted for 3 hours. In contrast, cells exposed to PMA in the presence of cholera toxin exhibited a marked recovery of binding within 60 minutes. The PMA-stimulated decrease in binding correlated with a rapid increase in the phosphorylation state of the EGF receptor. While phosphorylation of the receptor was sustained at an elevated level for at least three hours in cells receiving PMA alone, EGF receptor phosphorylation decreased between 1 and 3 hours in cells treated with PMA and cholera toxin. Furthermore, the cholera toxin-stimulated return of EGF binding was inhibited by treatment with the phosphatase inhibitor okadaic acid. These results suggest that a cholera toxin-activated phosphatase can increase binding capacity of the transmodulated EGF receptor in Rat-1 cells. Cholera toxin treatment elicited a qualitatively similar response in cells transmodulated by platelet-derived growth factor (PDGF). Okadaic acid antagonized the natural return of binding observed in cells stimulated with PDGF alone, indicating that a dephosphorylation event may be required for the recovery of normal EGF binding after receptor transmodulation. 相似文献
952.
Graeme D. Coles D. J. Abernethy Mary C. Christey A. J. Conner B. K. Sinclair 《Plant Molecular Biology Reporter》1991,9(1):13-20
Hairy roots, incited byAgrobacterium rhizogenes, form a useful system for analysing the expression and phenotypic effects of foreign genes in plant root tissue. Image analysis
offers a non-invasive method of describing their growth in culture. Images of pea (coarse) andBrassica (fine) hairy roots were captured, processed and analysed without difficulty using a commercially available image analysis
system. The value of this method in monitoring intermediate changes in growth pattern was illustrated by following the changes
in five putatively chlorsulfuron-resistantBrassicc hairy-root lines cultured with and without a selective level of chlorsulfuron. Areas of hairy-root research where this technique
will be particularly useful are discussed. 相似文献
953.
Mesoplodon peruvianus , a new species of beaked whale, is described on the basis of ten specimens which have either stranded or been captured between 11°12'S and 15°19'S latitude along the coasts of the provinces of Lima and Ica, south central Peru. This is the thirteenth living species of Mesoplodon recognized in the world's oceans. The animals that were examined were uniformly gray above, shading to lighter gray below. This whale is the smallest species of Mesoplodon (maximum body length 3.72 m) and is characterized by its teeth, which are small (31 to 65 mm long), ovate in cross section, and positioned 2.5% to 8.4% of the mandibular length from the anterior extremity, and posterior to the mandibular symphysis. 相似文献
954.
Data from the North Pacific sperm whale (Physeter catodon Linnaeus, 1758) fishery were examined for a possible density dependent change in growth during 40 yr harvesting after World War II. Early in this period males from the eastern stock were 16.8 m or less in length. By the early 1970s the largest males in the catch exceeded 16.8 m in length and reached 18.9 m in the late 1970s. The proportion of males measuring over 16.8 m, among sexually mature males (≥14.0 m), increased from 0 to >20% during the 1970s. Increases in the maximum size of males were possibly preceded by a change in the frequency distribution of body lengths in the middle 1960s when only 10% of the postwar catch had been taken. Testis weights suggested an increase in body length at sexual maturity. Two of the three putative North Pacific stocks showed similar growth changes. Adult males taken in the Bering Sea did not show such changes during the exploitation which ended in 1972 because of overfishing. Females showed no detectable change in body size. It is concluded that: (1) density dependent effects on male growth are greater before sexual maturity than after it, (2) males may show density dependent changes even at a population level above 90% of the carrying capacity, (3) polygynous males acquire more mates and realize higher reproductive success because of increased body size, and (4) females appear to maximize production by maturing earlier and shortening calving intervals in response to density change. 相似文献
955.
We have examined a serum-deprived culture system in order to verify that it is suitable for the study of burst forming unit erythroid (BFU-E) progenitor cells from premature neonates. Optimum growth of BFU-E from premature neonates was observed with each media constituent using the same concentration as that previously described for adult subjects. Growth of immature BFU-E from premature neonates were highly dependant upon a source of Burst Promoting Activity and mature BFU-E derived colonies emerged at day 12 compared to day 14 in adults. Our preliminary results with the validated medium suggest that premature infants have increased peripheral blood concentrations of BFU-E compared to healthy adult controls.Abbreviations Ad
Adherent cells
- BPA
Burst promoting activity
- BFU-E
Burst forming unit erythroid
- Epo
Erythropoietin
- IL3
Interleukin-3
- LDC
Low density (<1.077 g ml1) peripheral blood mononuclear cells 相似文献
956.
To determine the influence of the inoculum age on the kinetics of hybridoma growth and metabolism, spinner flasks have been inoculated with cells previously propagated in T flasks for 43, 52, 62 and 71 hr respectively. Increasing the age of the inoculum is found to result in a longer lag phase, in a lower maximum specific growth rate and in a reduced maximal cell density. During the growth phase specific rates of glucose and glutamine uptake and of ammonia and lactate production are similar. However, with the older inoculum, much higher metabolic activities are observed during the lag phase. The production of antibodies is delayed with increasing inoculum age, but the final antibody concentrations are similar, which indicates a higher specific antibody production rate when inoculating with older cells. 相似文献
957.
Jan-Kan Chen Ying-Tung Lau Jackson Chu 《In vitro cellular & developmental biology. Animal》1992,28(2):143-145
Summary Rat aortic endothelial cells were found to exhibit clonal variations in response to EGF stimulation in cell growth and prostacyclin
synthesis. EGF-induced growth and prostacyclin synthesis appeared to be regulated in a coordinated manner in that a clone
with a higher response to EGF growth stimulation also exhibited a higher response to EGF-stimulated prostacyclin synthesis.
This observation implys a possible involvement of prostacyclin synthesis in some of the biological effects of EGF on vascular
endothelial cells. 相似文献
958.
ERIC BAILLY RONAN JAMBOU JEAN SAVEL GINETTE JAUREGUIBERRY 《The Journal of eukaryotic microbiology》1992,39(5):593-599
ABSTRACT We investigated the effect of a cysteine proteinase inhibitor (E-64) and an aspartyl proteinase inhibitor (Pepstatin A) on asexual erythrocytic stages of Plasmodium falciparum in culture. These two protease inhibitors showed different patterns of activity. E-64 acted preferentially against trophozoite and schizont stages. After 48 h incubation at high concentrations of E-64 (28, 140, 280 μM), growth was totally abolished and the parasites presented characteristic enlarged food vacuoles. Morphological alterations were also seen after shorter incubation periods (6 h at 28 μM) or 12 h at the inhibitory concentration 50% (12 μM), but an additional culture period (24 h) in inhibitor-free medium allowed normal parasite development, demonstrating a parasitostatic effect. E-64 acts on parasite multiplication; the normal merozoite maturation was altered and the normal reinvasion process partially impaired. Pepstatin A used at the inhibitory concentration 50% (4 μM) killed the parasites before trophozoite development and had a major effect on schizonts maturation. No altered parasite development occurred during an additional culture period without Pepstatin A, demonstrating a parasiticidal effect. E-64 and Pepstatin A used in combination inhibit the parasite growth with a strong synergistic effect. 相似文献
959.
H. J. G. ten Hoopen W. M. van Gulik J. J. Heijnen 《In vitro cellular & developmental biology. Plant》1992,28(3):115-120
Summary Continuous culture is an attractive research tool in physiologic and growth and production kinetics research. However, fulfillment
of the basic assumptions of continuous culture in the experimental set-up may cause problems. The homogeneity of plant cell
cultures and effluent, particularly, may cause problems. This paper presents an experimental set-up which solves these problems
and describes the use of this equipment in a study of the growth kinetics of plant cells. Industrial application of the continuous
culture of plant cells in the production of secondary metabolites seems to be profitable when compared with batch or fed-batch
cultures. However, various problems such as uncoupled product formation and strain instability make fed-batch culture a better
choice.
Presented in the Session-in-Depth Batch Production and Fermentation at the 1991 World Congress on Cell and Tissue Culture,
Anaheim, California, June 16–20, 1991. 相似文献
960.
毛百合繁殖生物学研究——Ⅰ 毛百合的自然生长与繁殖 总被引:2,自引:0,他引:2
本文对产于东北地区的毛百合进行了自然状况下的生长与繁殖研究。结果发现在不同的生境下毛百合的生长发育有三种类型,即生长旺盛类型;可完成生活史,但生长受到一定限制类型;不能完成生活史,只能营养生长类型。 在毛百合完成其生活史的转化中光是一个重要限制因子。在全光的46.6%的生境中毛百合尚能大部向成株转化,并开花结果完成生活史,而在相对光照为15.6%的林下,毛百合转化为成株的概率很少。 由于长期适应环境的结果,毛百合具有两种繁殖对策,一是在全光下或不低于相对光照的46%的生境下,有性与无性繁殖同时进行;而在低于相对光照的15%以下时,除个别光强处外,毛百合主要以鳞茎繁殖和生长,一旦林地受干扰后,则马上开花结实,营有性繁殖。 相似文献